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Diazepam works by enhancing the effects of gamma-aminobutyric acid (GABA), a neurotransmitter in the brain. GABA serves as an inhibitory neurotransmitter that reduces neuronal excitability throughout the nervous system. By increasing GABA activity, diazepam effectively calms the brain, leading to its anxiolytic (anxiety-reducing), anticonvulsant, muscle relaxant, and hypnotic (sleep-inducing) properties.